Simultaneous Determination of Five Effective Components in HuangdiAnxiao Capsules by UPLC
LI Yu-xin1,2,WANG Yong-zhong1,WU Xiao-ming1,XIA Lun-zhu1,WEI Liang-bing1,SHAN Li1,HAN Yan-quan1*
Author information+
1.The First Affiliated Hospital, Anhui University of Chinese Medicine, Grade 3 Laboratory of TCM Preparation, State Administration of Anhui University of Chinese Medicine, Hefei 230038,China;
2. Anhui University of Chinese Medicine, Hefei 230031,China
OBJECTIVE To establish a UPLC method for simultaneous determination of puerarin, daidzin, daidzine, palmatine, and berberine in HuangdiAnxiao capsules(HDAXC). METHODS The chromatographic analyses were carried out on an ACQUITY UPLCTM BEH C18 column (2.1 mm×100 mm, 1.7 μm; Waters Corp., USA) maintained at 30 ℃.The mobile phase component A was acetonitrile and B was phosphoric acid with gradient elution at 0.2 mL·min-1.The five components were detected by dual wavelength, ie λ1=250 nm,λ2=354 nm. RESULTS The standard curves of puerarin, daidzin, daidzine, palmatine, and berberine showed good linear relationship in the ranges of 0.022-0.176 μg(r=0.999 9), 0.004 3-0.034 4 μg(r=0.999 9), 0.000 61-0.004 9 μg(r=0.999 7), 0.002 3-0.018 4 μg(r=0.999 7), and 0.008 6-0.066 8 μg(r=0.999 6),respectively. The average recoveries were 97.70%, 96.81%, 99.36%, 99.60% and 98.67% with RSDs of less than 2.7%(n=6). CONCLUSION This method is simple, rapid, accurate, and reproducible, and it can lay basis for the quality evaluation of HDAXC.
LI Yu-xin,,WANG Yong-zhong,WU Xiao-ming,XIA Lun-zhu,WEI Liang-bing,SHAN Li,HAN Yan-quan*.
Simultaneous Determination of Five Effective Components in HuangdiAnxiao Capsules by UPLC[J]. Chinese Pharmaceutical Journal, 2015, 50(4): 362-365 https://doi.org/10.11669/cpj.2015.04.021
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